Techniques to detect toxicity (accessed by) :
(a) Protein Characterization
(b) Digestive fate in simulated gastric and intestinal fluids
(c) Acute oral toxicity
(d) Amino acid / nucloetide homology
(e) Rely on heuristic ( assumptions based )
(f) Conduct acute oral toxicity of the refined target protein ( only ) , simulated digestion od the target protein ( only ) , plus heuristic reasoning

-http://www.plant.uoguelph.ca/research/homepages/eclark/safety.htm